借助Nextera技术,DNA可在单管酶反应中同时片段化并加上测序接头。Nextera XT试剂盒支持仅1纳克的超低DNA输入,因此可支持众多输入样本(对于扩增子输入,长度至少需为300碱基对)。
对于需要更高通量的项目而言,还可对每个Nextera XT文库多达384个样本进行多重分析。另外,Nextera XT试剂盒还引入了基于微珠的创新型样本标准化,从而消除了在混合和测序之前进行文库定量的需要。使用Nextera XT试剂盒制备的文库与Illumina所有的测序仪兼容。
| Assay Time | 90 minutes |
|---|---|
| Hands-On Time | 15 minutes |
| Mechanism of Action | Enzymatic Fragmentation |
| Multiplexing | Up to 384 uniquely indexed samples may be pooled and sequenced together. |
| Input Quantity | 1 ng DNA |
| Species | Any Species, Bacteria, Human, Mammalian, Mouse, Plant, Rat, Yeast |
| Method | 16S rRNA Sequencing, Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Targeted DNA Sequencing, Whole-Genome Sequencing |
| Variant Class | Single Nucleotide Polymorphisms (SNPs), Structural Variants |
| System Compatibility | MiniSeq, MiSeq, MiSeq FGx in Research Mode, NextSeq 500, NextSeq 550 |
| Specialized Sample Types | Low Input, Single Cells |
| Technology | Sequencing |
| Automation Capability | Liquid Handling Robots |